The three embryonic stem cell lines described on the following pages, RUES1, RUES2 and RUES3, are available to the research community upon completion of the order process at www.wicell.org/rues.

   

RUES1 (NIH Registration number 0012)

Biosafety Level: 2

Shipped: Frozen

Medium & Serum / Propogation / Subculturing / Preservation: See the Protocol listed on WiCell’s RUES1 page

Organism: Homo sapiens (human)

Morphology: Spherical colony

Cell Type: Human embryonic stem cell

Requests: To request cells, go to the RUES1 page on the WiCell website and complete the order process.

Restrictions: Cannot be used for diagnostic or therapeutic use; for use in teaching and non-commercial research only.

Cytogenetic Analysis: XY

Age: embryo, blastocyst

Comments: RUES1 is a human embryonic stem cell line derived from a de-identified frozen embryo that was originally generated for reproductive purposes and later donated with informed consent for research purposes. The Provider does not have any information that could be used to identify the embryo and/or gamete donors. RUES1 cells are pluripotent and can differentiate to representatives of the three primary germ layers. See RUES1 Derivation Information

References: Recipients are requested to provide notice of publications citing use of this line to the Provider.

Notices and Disclaimers

The Material is experimental in nature and must be used with prudence and appropriate caution, since not all of its characteristics are known. The Material is provided without warranty of merchantability or fitness for a particular purpose or any other warranty, express or implied. Provider makes no representation or warranty that the use of the Material will not infringe any patent or other proprietary right. The Material cannot be used for any commercial purpose or for work involving human subjects, including diagnostic testing.

RUES2 (NIH Registration number 0013)

Biosafety Level: 2

Shipped: Frozen

Medium & Serum / Propogation / Subculturing / Preservation: See the Protocol listed on WiCell’s RUES2 page

Organism: Homo sapiens (human)

Morphology: Spherical colony

Cell Type: Human embryonic stem cell

Requests: To request cells, go to the RUES2 page on the WiCell website and complete the order process.

Restrictions: Cannot be used for diagnostic or therapeutic use; for use in teaching and non-commercial research only.

Cytogenetic Analysis: XX

Age: embryo, blastocyst

Comments: RUES2 is a human embryonic stem cell line derived from a de-identified frozen embryo that was originally generated for reproductive purposes and later donated with informed consent for research purposes. The Provider does not have any information that could be used to identify the embryo and/or gamete donors. RUES2 cells are pluripotent and can differentiate to representatives of the three primary germ layers. See RUES2 Derivation Information

References: Recipients are requested to provide notice of publications citing use of this line to the Provider.

Notices and Disclaimers

The Material is experimental in nature and must be used with prudence and appropriate caution, since not all of its characteristics are known. The Material is provided without warranty of merchantability or fitness for a particular purpose or any other warranty, express or implied. Provider makes no representation or warranty that the use of the Material will not infringe any patent or other proprietary right. The Material cannot be used for any commercial purpose or for work involving human subjects, including diagnostic testing.

RUES3 (NIH Registration number 0042)

Biosafety Level: 2

Shipped: Frozen

Medium & Serum / Propogation / Subculturing / Preservation: See the Protocol listed on WiCell’s RUES3 page

Organism: Homo sapiens (human)

Morphology: Spherical colony

Cell Type: Human embryonic stem cell

Requests: To request cells, go to the RUES3 page on the WiCell website and complete the order process.

Restrictions: Cannot be used for diagnostic or therapeutic use; for use in teaching and non-commercial research only.

Cytogenetic Analysis: XY

Age: embryo, blastocyst

Comments: RUES3 is a human embryonic stem cell line derived from a de-identified frozen embryo that was originally generated for reproductive purposes and later donated with informed consent for research purposes. The Provider does not have any information that could be used to identify the embryo and/or gamete donors. RUES3 cells are pluripotent and can differentiate to representatives of the three primary germ layers.

References: Recipients are requested to provide notice of publications citing use of this line to the Provider.

Notices and Disclaimers

The Material is experimental in nature and must be used with prudence and appropriate caution, since not all of its characteristics are known. The Material is provided without warranty of merchantability or fitness for a particular purpose or any other warranty, express or implied. Provider makes no representation or warranty that the use of the Material will not infringe any patent or other proprietary right. The Material cannot be used for any commercial purpose or for work involving human subjects, including diagnostic testing.

RUES Sample Request

RUES1, RUES2, and RUES3 are available from WiCell. To request cells, visit www.wicell.org/rues on the WiCell website and complete the order process. Information regarding the order process is available at www.wicell.org/orderoverview.